Rationelle Beckenkamm-Biopsie

Summary Unfortunately, the combination of the superior histologic embedding technique for semi-thin sectioning of undecalcified bone in methacrylate with special methods in enzyme or immune histochemistry did not prove to be very successful up to now. These shortcomings are attributed to the influen...ce of the embedding medium itself. They can be overcome by processing one part of a biopsy specimen after embedding in methacrylate, the other one after freezing. Optimal and comparable results have been obtained by a simple instrument for longitudinal hemisectioning of a cylindrical biopsy specimen of cancellous bone of adequate size, and by developing a suitable technique for the cutting of frozen sections of undecalcified bone. These methods are presented. Examples are given of the benefits of the simultaneous evaluation of one and the same biopsy-specimen with light-microscopic, ultramicroscopic, enzyme- and immun-histochemic technique. Ausführliche Beschreibung Beschreibung ausblenden

1. Person:	Bartl, R.
Weitere Personen:	Burkhardt, R.; Vondracek, H.; Sommerfeld, W.; Hagemeister, E.
Quelle:	in Journal of molecular medicine Vol. 56 (1978), p. 545-550 Weitere Artikel
Format:	Online-Artikel
Genre:	Histochemistry, Immunohistology, Plastic embedding, Frozen sections, Bone biopsy, Bone marrow, Histochemie, Immunhistologie, Kunststoffeinbettung, Gefrierschnitte, Myelotomie, Knochenbiopsie, Knochenmark
Sprache:	German
Veröffentlicht:	1978
Beschreibung:	Online-Ressource
Online Zugang:	Online
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Anmerkung:	Copyright: Copyright 1978 Springer-Verlag