Interaction of murine progesterone receptors with specific monoclonal antibodies to the avian progesterone receptor

Monoclonal antibodies raised against purified chicken progesterone receptor (PgR) have been described and characterized recently. In this study we have screened these antibodies for cross-reactivity with murine PgR. Of the six anti-PgR antibodies tested, one (αPR6) precipitates murine PgR in an ass... Ausführliche Beschreibung

1. Person: Schneider, W.
Weitere Personen: Toft, D.O.; Sullivan, W.P.; Shyamala, G.
Quelle: in Journal of Steroid Biochemistry Vol. 29, No. 3 (1988), p. 297-306
Weitere Artikel
Format: Online-Artikel
Sprache: English
Veröffentlicht: 1988
Beschreibung: Online-Ressource
Online Zugang: Online
Volltext
Tags: Hinzufügen
Keine Tags. Fügen Sie den ersten Tag hinzu!
Anmerkung: Copyright: Copyright (c) 2002 Elsevier Science B.V.
LEADER 02103nma a2200349 c 4500
001 NLEJ186380143
003 DE-601
005 20151015032024.0
007 cr uuu---uuuuu
008 070506s1988 000 0 eng d
016 7 |a 2198730-0  |2 DE-600 
024 8 |a 0022-4731(88)90030-1 
035 |a (DE-599)GBVNLZ186380143 
040 |b ger  |c GBVCP 
041 0 |a eng 
084 |a sdj  |2 natliz 
245 0 0 |a Interaction of murine progesterone receptors with specific monoclonal antibodies to the avian progesterone receptor  |h Elektronische Ressource 
300 |a Online-Ressource 
500 |a Copyright: Copyright (c) 2002 Elsevier Science B.V. 
520 |a Monoclonal antibodies raised against purified chicken progesterone receptor (PgR) have been described and characterized recently. In this study we have screened these antibodies for cross-reactivity with murine PgR. Of the six anti-PgR antibodies tested, one (αPR6) precipitates murine PgR in an assay using protein A-sepharose as an absorbent for the antibody. The antibody is specific for PgR and does not react with the estrogen receptor or the glucocorticoid receptor in the same cytosol. In immunoblot experiments, both αPR6 and αPR11 recognize a 115,000 Da protein, however, α PR11 gives a weaker signal than αPR6. In photoaffinity labeling experiments, a 115,000 Da and an 83,000 Da protein covalently bind tritiated R5020 in a receptor-specific way. We conclude that the αPR6 antibody can be used as a tool to study the structure and function of the murine PgR. 
533 |a Online edition  |f Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 ; 041023-8 
700 1 |a Schneider, W. 
700 1 |a Toft, D.O. 
700 1 |a Sullivan, W.P. 
700 1 |a Shyamala, G. 
773 0 8 |i in  |t Journal of Steroid Biochemistry  |d Amsterdam : Elsevier  |g Vol. 29, No. 3 (1988), p. 297-306  |q 29:3<297-306  |w (DE-601)NLEJ18527725X  |x 0022-4731 
856 4 0 |u http://linkinghub.elsevier.com/retrieve/pii/0022-4731(88)90030-1 
901 |a OLC 
912 |a ZDB-1-SDJ 
912 |a GBV_NL_ARTICLE 
951 |a AR 
952 |d 29  |j 1988  |e 3  |h 297-306 

Ähnliche Einträge

Keine ähnlichen Titel gefunden

Privacy Notice Ask a Librarian New Acquisitions