A new acidic protein in porcine brain

An extremely acidic protein has been isolated in a purified form from porcine brain extract, by (NH"4)"2SO"4 fractionation followed by column chromatography on DEAE-Sephadex A-50 and on Sephadex G-75. The purified protein was tentatively named as glutamic acid-rich protein because it was characteriz... Ausführliche Beschreibung

1. Person: Ishioka, N.
Weitere Personen: Isobe, T.; Okuyama, T.; Numata, Y.; Wada, H.
Quelle: in BBA - Protein Structure Vol. 625, No. 2 (1980), p. 281-290
Weitere Artikel
Format: Online-Artikel
Genre: Acidic protein purification, Glutamic acid, (Porcine brain)
Sprache: English
Veröffentlicht: 1980
Beschreibung: Online-Ressource
Online Zugang: Online
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Anmerkung: Copyright: Copyright (c) 2002 Elsevier Science B.V.
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520 |a An extremely acidic protein has been isolated in a purified form from porcine brain extract, by (NH"4)"2SO"4 fractionation followed by column chromatography on DEAE-Sephadex A-50 and on Sephadex G-75. The purified protein was tentatively named as glutamic acid-rich protein because it was characterized by its remarkably high content of glutamic acid which accounted for 49% of the total amino acid composition. The protein appeared to be a single polypeptide chain with a molecular weight of 56 000-58 000, and had an isoelectric point of 4.6. The N-terminal amino acid sequence was Asp-Glu-Pro-Pro-Ser-GluGly. The immunochemical analysis using rabbit antiserum prepared to the porcine protein has suggested that it is present in the brain of human, cow, cat, dog and goat as well as in various goat organs including liver, kidney, heart, small intestine and spleen. 
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700 1 |a Wada, H. 
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