Esterification of cholesterol by rat adrenal gland homogenates and subcellular components

This study demonstrates that the rat adrenal contains two enzymes that esterify cholesterol: one present principally in the mitochondria-rich fraction, the other most active in the microsome-rich fraction. The enzyme in the microsome-rich fraction had an optimum activity at pH 6.6 and required the a... Ausführliche Beschreibung

1. Person: Shyamala, G.
Weitere Personen: Lossow, W.J.; Chaikoff, I.L.
Quelle: in Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism Vol. 116, No. 3 (1966), p. 543-554
Weitere Artikel
Format: Online-Artikel
Sprache: English
Veröffentlicht: 1966
Beschreibung: Online-Ressource
Online Zugang: Online
Volltext
Tags: Hinzufügen
Keine Tags. Fügen Sie den ersten Tag hinzu!
Anmerkung: Copyright: Copyright (c) 2002 Elsevier Science B.V.
Zusammenfassung: This study demonstrates that the rat adrenal contains two enzymes that esterify cholesterol: one present principally in the mitochondria-rich fraction, the other most active in the microsome-rich fraction. The enzyme in the microsome-rich fraction had an optimum activity at pH 6.6 and required the addition of ATP, CoA, Mg^2^+ and GSH for its activity. The enzyme in the mitochondria-rich fraction had an optimum activity at pH 5.0 and did not require the addition of these cofactors, but required cell sap or a lipid extract of cell sap for its activity. At least one of the ways in which cell sap exerted this stimulatory effect was by providing both free cholesterol and the fatty acid for the esterification. Both triolein and oleic acid served as the source of fatty acid for the esterification by the enzyme associated with the mitochondria-rich fraction, but the triolein was apparently hydrolyzed to free fatty acids before incorporation into the esters. Neither 2,4-dinitrophenol nor sodium arsenate inhibited the esterification, thereby suggesting that ATP was not involved in the activation of fatty acids before the esterification of cholesterol by that fraction of the rat adrenal gland.
ISSN: 0005-2760

Ähnliche Einträge

Keine ähnlichen Titel gefunden

Privacy Notice Ask a Librarian New Acquisitions