A microassay system for measuring esterase activity and protein concentration in small samples and in high-pressure liquid chromatography eluate fractions

A microtiter plate spectrophotometric system has been used together with the Bradford, Ellman, and van Asperen assays to measure protein concentration (to 0.5 μg) and the activities of acetylcholinesterase (to 10^-^3 units) and carboxylesterase (to 30 μg β-napthol reaction product) in small sampl... Ausführliche Beschreibung

1. Person: Brogdon, W.G.
Weitere Personen: Dickinson, C.M.
Quelle: in Analytical Biochemistry Vol. 131, No. 2 (1983), p. 499-503
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Format: Online-Artikel
Sprache: English
Veröffentlicht: 1983
Beschreibung: Online-Ressource
Online Zugang: Online
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Anmerkung: Copyright: Copyright (c) 2005 Elsevier (USA)
Zusammenfassung: A microtiter plate spectrophotometric system has been used together with the Bradford, Ellman, and van Asperen assays to measure protein concentration (to 0.5 μg) and the activities of acetylcholinesterase (to 10^-^3 units) and carboxylesterase (to 30 μg β-napthol reaction product) in small samples such as high-performance liquid chromatographic eluate fractions. For 100-μl samples, at least 300 Ellman acetylcholinesterase or Bradford protein assays can be conducted and read in less than 30 min, and a like number of van Asperen nonspecific esterase assays (including 1-h incubation) run in less than 90 min. The eluate from a single 20-min high-performance liquid chromatographic run of a biological sample can be collected as up to 300 fractions directly into microtiter plate wells, and the three assays run on all fractions in less than 90 min.
ISSN: 0003-2697

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