Methane formation from acetyl phosphate in cell extracts of Methanosarcina barkeri Dependence of the reaction on coenzyme A

Cell extracts (100 000 x g supernatant) of acetate grown Methanosarcina barkeri (strain MS) catalyzed the formation of methane from acetyl phosphate (apparent K"m ~ 2 mM) with specific activities up to 50 nmol.min^-^1.mg protein^-^1, which is 25% of the specific rate of methane formation from acetat... Ausführliche Beschreibung

1. Person: Fischer, R.
Weitere Personen: Thauer, R.K.
Quelle: in FEBS Letters Vol. 228, No. 2 (1988), p. 249-253
Weitere Artikel
Format: Online-Artikel
Genre: Acetyl-CoA, Acetyl phosphate, Coenzyme A, Methanogenesis, Coenzyme M, (Methanosarcina barkeri)
Sprache: English
Veröffentlicht: 1988
Beschreibung: Online-Ressource
Online Zugang: Online
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Anmerkung: Copyright: Copyright (c) 2002 Federation of European Biochemical Societies
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520 |a Cell extracts (100 000 x g supernatant) of acetate grown Methanosarcina barkeri (strain MS) catalyzed the formation of methane from acetyl phosphate (apparent K"m ~ 2 mM) with specific activities up to 50 nmol.min^-^1.mg protein^-^1, which is 25% of the specific rate of methane formation from acetate by intact cells. Methane formation from acetyl phosphate was strictly dependent on coenzyme A (CoA) (apparent K"m ~ 0.1 mM), which was demonstrated with extracts pretreated with charcoal to remove endogenous CoA. The extracts contained high specific activities of acetate kinase (16 μmol.min^-^1.mg protein^-^1) and of phosphotransacetylase (26 μmol.min^-^1.mg protein^-^1). The findings indicate that methanogenesis from acetate proceeds via acetyl phosphate and acetyl-CoA as intermediates. Almost identical results were obtained with cell extracts of acetate grown M. barkeri (strain Fusaro). 
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