ATPases and Phosphate Exchange Activities in Magnesium Chelatase Subunits of Rhodobacter sphaeroides

Three separate proteins, BchD, BchH, and BchI, together with ATP, insert magnesium into protoporphyrin IX. An analysis of ATP utilization by the subunits revealed the following: BchH catalyzed ATP hydrolysis at the rate of 0.9 nmol per min per mg of protein. BchI and BchD, tested individually, had n... Ausführliche Beschreibung

1. Person: Hansson, Mats
Weitere Personen: Kannangara, C. Gamini verfasserin
Quelle: in Proceedings of the National Academy of Sciences of the United States of America Vol. 94, No. 24 (1997), p. 13351-13356
Weitere Artikel
Format: Online-Artikel
Sprache: English
Veröffentlicht: 1997
Beschreibung: Online-Ressource
Schlagworte: research-article
Plant Biology
Chlorophyll
BchD
BchH
BchI
Phosphorylation
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Anmerkung: Copyright: Copyright 1993-1997 National Academy of Sciences
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245 1 0 |a ATPases and Phosphate Exchange Activities in Magnesium Chelatase Subunits of Rhodobacter sphaeroides  |h Elektronische Ressource 
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520 |a Three separate proteins, BchD, BchH, and BchI, together with ATP, insert magnesium into protoporphyrin IX. An analysis of ATP utilization by the subunits revealed the following: BchH catalyzed ATP hydrolysis at the rate of 0.9 nmol per min per mg of protein. BchI and BchD, tested individually, had no ATPase activity but, when combined, hydrolyzed ATP at the rate of 117.9 nmol/min per mg of protein. Magnesium ions were required for the ATPase activities of both BchH and BchI+D, and these activities were inhibited 50% by 2 mM o-phenanthroline. BchI additionally catalyzed a phosphate exchange reaction from ATP and ADP. We conclude that ATP hydrolysis by BchI+D is required for an activation step in the magnesium chelatase reaction, whereas ATPase activity of BchH and the phosphate exchange activity of BchI participate in subsequent reactions leading to the insertion of Mg<sup>2+</sup> into protoporphyrin IX. 
653 |a research-article 
653 |a Plant Biology 
653 |a Chlorophyll 
653 |a BchD 
653 |a BchH 
653 |a BchI 
653 |a Phosphorylation 
700 1 |a Kannangara, C. Gamini  |e verfasserin  |4 aut 
773 0 8 |i in  |t Proceedings of the National Academy of Sciences of the United States of America  |d Washington, DC : National Acad. of Sciences  |g Vol. 94, No. 24 (1997), p. 13351-13356  |q 94:24<13351-13356  |w (DE-601)JST069399220  |x 0027-8424 
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952 |d 94  |j 1997  |e 24  |h 13351-13356 

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