Influenza Viral (A/WSN/33) Hemagglutinin is Expressed and Glycosylated in the Yeast Saccharomyces cerevisiae

Recombinant plasmids were constructed in which genes coding for either the entire or the signal-minus (amino acid residues 2-17 deleted) hemagglutinin (HA) of WSN influenza virus were placed under the control of the alcohol dehydrogenase I gene promoter of Saccharomyces cerevisiae. Both recombinant ... Ausführliche Beschreibung

1. Person: Jabbar, M. Abdul
Weitere Personen: Sivasubramanian, N. verfasserin; Nayak, Debi P. verfasserin
Quelle: in Proceedings of the National Academy of Sciences of the United States of America Vol. 82, No. 7 (1985), p. 2019-2023
Weitere Artikel
Format: Online-Artikel
Sprache: English
Veröffentlicht: 1985
Beschreibung: Online-Ressource
Schlagworte: research-article
Biochemistry
Recombinant DNA
Shuttle Vector
Alcohol Dehydrogenase I Promoter
Glycosylation
Online Zugang: Volltext
Volltext
Tags: Hinzufügen
Keine Tags. Fügen Sie den ersten Tag hinzu!
Zusammenfassung: Recombinant plasmids were constructed in which genes coding for either the entire or the signal-minus (amino acid residues 2-17 deleted) hemagglutinin (HA) of WSN influenza virus were placed under the control of the alcohol dehydrogenase I gene promoter of Saccharomyces cerevisiae. Both recombinant plasmids were shown to direct the synthesis of HA-specific polypeptides that were detected by immunoprecipitation with antiviral antibodies. The complete HA produced in yeast had an approximate Mrof 70,000 and was glycosylated, as determined by the endoglycosidase H sensitivity, and was bound to membrane. Therefore, the complete HA polypeptide possessing the signal sequence probably traversed the yeast secretory pathways. Signal-minus HA, on the other hand, had a lower molecular weight and was nonglycosylated. The specific binding of yeast HA with antiviral antibodies could be competitively inhibited by influenza viral HA, demonstrating that the HA produced in yeast contained antigenic determinants of the native viral HA.
ISSN: 0027-8424

Ähnliche Einträge

Keine ähnlichen Titel gefunden

Privacy Notice Ask a Librarian New Acquisitions