Role of Surface Modulating Assemblies in Growth Control of Normal and Transformed Fibroblasts

Cellular microtubules, microfilaments, and surface receptors have been postulated to form a surface modulating assembly that regulates surface receptor mobility and cell growth. To test this hypothesis, we examined three agents known to affect cell growth [colchicine, concanavalin A (Con A), and the... src gene product of Rous sarcoma virus] for their effects on chick embryo fibroblasts. Individual cells from serum-starved normal fibroblast populations became committed to enter S phase at various times over a 12 hr period after exposure to serum. Colchicine and other microtubule-disrupting agents blocked entry into S phase at a point close to the commitment point for each cell. The lectin Con A also blocked entry into the S phase when present in doses sufficient to modulate surface receptor mobility. In contrast, succinyl-Con A, which does not induce surface modulation, had no effect. Both Con A and colchicine blocked the appearance of cytoplasmic factors capable of stimulating DNA replication in a cell-free system. To study endogenous effects on the surface modulating assembly, we infected fibroblasts with a Rous sarcoma virus (tsNY68) having a temperature-sensitive mutation in the transforming (src) gene. We have previously shown that microtubular and microfilamentous structures o Ausführliche Beschreibung Beschreibung ausblenden

1. Person:	McClain, Donald A.
Weitere Personen:	D'Eustachio, Peter verfasserin; Edelman, Gerald M. verfasserin
Quelle:	in Proceedings of the National Academy of Sciences of the United States of America Vol. 74, No. 2 (1977), p. 666-670 Weitere Artikel
Format:	Online-Artikel
Sprache:	English
Veröffentlicht:	1977
Beschreibung:	Online-Ressource
Schlagworte:	research-article Cell biology Rous sarcoma virus Concanavalin A Microtubules Cell cycle
Online Zugang:	Volltext
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