ATP Regulates the Differentiation of Mammalian Skeletal Muscle by Activation of a P2 X₅ Receptor on Satellite Cells

ATP is well known for its role as an intracellular energy source. However, there is increasing awareness of its role as an extracellular messenger molecule (Burnstock, 1997). Although evidence for the presence of receptors for extracellular ATP on skeletal myoblasts was first published in 1983 (Kolb... and Wakelam), their physiological function has remained unclear. In this paper we used primary cultures of rat skeletal muscle satellite cells to investigate the role of purinergic signaling in muscle formation. Using immunocytochemistry, RT-PCR, and electrophysiology, we demonstrate that the ionotropic P2 X₅ receptor is present on satellite cells and that activation of a P2X receptor inhibits proliferation, stimulates expression of markers of muscle cell differentiation, including myogenin, p21, and myosin heavy chain, and increases the rate of myotube formation. Furthermore, we demonstrate that ATP application results in a significant and rapid increase in the phosphorylation of MAPKs, particularly p38, and that inhibition of p38 activity can prevent the effect of ATP on cell number. These results not only demonstrate the existence of a novel regulator of skeletal muscle differentiation, namely ATP, but also a new role for ionotropic P2X receptors in the control of cell f Ausführliche Beschreibung Beschreibung ausblenden

1. Person:	Ryten, Mina
Weitere Personen:	Dunn, Philip M. verfasserin; Neary, Joseph T. verfasserin; Burnstock, Geoffrey verfasserin
Quelle:	in The journal of cell biology : JCB Vol. 158, No. 2 (2002), p. 345-355 Weitere Artikel
Format:	Online-Artikel
Sprache:	English
Veröffentlicht:	2002
Beschreibung:	Online-Ressource
Schlagworte:	research-article ATP P2 X<sub>5</sub> Skeletal muscle Differentiation Satellite cells
Online Zugang:	Volltext
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Anmerkung:	Copyright: Copyright 2002 The Rockefeller University Press