Chemically Defined Serum-Free Media for the Cultivation of Primary Cells and Their Susceptibility to Viruses

Chemically defined media SFRE-199-1 for the growth and SFRE-199-2 for the maintenance of primary baboon kidney (Bak) cell cultures were formulated by supplementing medium M199 with insulin, sodium pyruvate, zinc sulfate, and increasing arginine-HCl, cysteine, cystine, L-glutamine, L-glutamic acid, g... Ausführliche Beschreibung

1. Person: Kalter, S. S. verfasserin
Weitere Personen: Heberling, R. L. verfasserin
Quelle: in In vitro : cellular & developmental biology : journal of the Tissue Culture Association Vol. 16, No. 7 (1980), p. 616-628
Weitere Artikel
Format: Online-Artikel
Sprache: English
Veröffentlicht: 1980
Beschreibung: Online-Ressource
Schlagworte: research-article
Chemically Defined Medium
Primary Baboon Kidney Cells (Bak)
Selenium
Protamine Zinc Insulin (PZI)
Galactose
Amino Acids
Zinc Sulfate
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Anmerkung: Copyright: Copyright 1980 Tissue Culture Association
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520 |a Chemically defined media SFRE-199-1 for the growth and SFRE-199-2 for the maintenance of primary baboon kidney (Bak) cell cultures were formulated by supplementing medium M199 with insulin, sodium pyruvate, zinc sulfate, and increasing arginine-HCl, cysteine, cystine, L-glutamine, L-glutamic acid, glycine, histidine, tyrosine, and glucose to maximally active nontoxic concentrations. For prolonged maintenance of the cells, physiological pH control, and blocking of excessive lactic acid accumulation in the spent medium of the cell cultures, it was necessary to supplement the medium containing Earle's balanced salts with D-(+) galactose. The cells grew and were maintained equally well on glass or polystyrene surfaces. Selenium, when added to growth medium or substituted for insulin and zinc sulfate, did not stimulate cell growth. Electron microscopy showed that numerous dense particles, approximately 250 to 400 Å in diameter, with the appearance of glycogen, were found throughout the cytoplasm in the cells grown in SFRE-199-1 and maintained in SFRE-199-2. Echovirus types 1 to 3, poliovirus types 1 to 3, coxsackievirus types B2, B4, B5, Herpesvirus hominis type 1, simian herpesvirus H. simiae and SA8, and simian adenovirus SV34 when titrated in primary Bak cells and grown and maintained in SFRE-199-1 and 2, respectively, developed titers comparable to those obtained in conventionally grown and maintained cells. 
653 |a research-article 
653 |a Chemically Defined Medium 
653 |a Primary Baboon Kidney Cells (Bak) 
653 |a Selenium 
653 |a Protamine Zinc Insulin (PZI) 
653 |a Galactose 
653 |a Amino Acids 
653 |a Zinc Sulfate 
700 1 |a Kalter, S. S.  |e verfasserin  |4 aut 
700 1 |a Heberling, R. L.  |e verfasserin  |4 aut 
773 0 8 |i in  |t In vitro : cellular & developmental biology : journal of the Tissue Culture Association  |d Gaithersbury, Md  |g Vol. 16, No. 7 (1980), p. 616-628  |q 16:7<616-628  |w (DE-601)JST041876989  |x 0073-5655 
856 4 1 |u https://www.jstor.org/stable/4292389  |3 Volltext 
912 |a GBV_JSTOR 
951 |a AR 
952 |d 16  |j 1980  |e 7  |h 616-628 

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