Individual Human Tumors in Short-Term Micro-Organ Cultures: Chemosensitivity Testing by Fluorescent Cytoprinting

Using innovative approaches, we addressed several problems often associated with in vitro chemosensitivity testing of individual human tumors: 1) obtaining a high rate of evaluability; 2) excluding participation of nonmalignant stromal and vascular components usually present in tumor specimens; 3) p... Ausführliche Beschreibung

1. Person: Rotman, Boris
Weitere Personen: Dickinson, Katherine verfasserin
Quelle: in In vitro : cellular & developmental biology : journal of the Tissue Culture Association Vol. 24, No. 11 (1988), p. 1137-1146
Weitere Artikel
Format: Online-Artikel
Sprache: English
Veröffentlicht: 1988
Beschreibung: Online-Ressource
Schlagworte: research-article
Human Tumor
In vitro
Chemotherapy
Organ Culture
Cytotoxicity
Online Zugang: Volltext
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Anmerkung: Copyright: Copyright 1988 Tissue Culture Association
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245 1 0 |a Individual Human Tumors in Short-Term Micro-Organ Cultures: Chemosensitivity Testing by Fluorescent Cytoprinting  |h Elektronische Ressource 
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520 |a Using innovative approaches, we addressed several problems often associated with in vitro chemosensitivity testing of individual human tumors: 1) obtaining a high rate of evaluability; 2) excluding participation of nonmalignant stromal and vascular components usually present in tumor specimens; 3) preserving cell-to-cell interactions present in the original tumor; 4) assessing drug-induced cytotoxicity without sacrificing the tumor culture. To circumvent these problems, tumor specimens were processed as follows: i) tissue (fresh or cryopreserved) was mechanically or enzymatically dissociated under mild conditions into cellular clusters (termed micro-organs); ii) large micro-organs were separated by a brief decantation, resuspended, and then exposed to fluorescein acetate to visualize (under naked eye) viable micro-organs; iii) fluorescent (i.e., viable) micro-organs were collected using a Pasteur pipette, and then planted on a solid support made of cellulose fibers impregnated with collagen. Since tumor micro-organs have been previously shown to consist solely of malignant cells, the procedure described here not only preserves a critical portion of the tumor architecture but eliminates at the onset necrotic tissue and nonmalignant cellular components that could interfere with the chemosensitivity testing. Drug-induced cytotoxicity was measured by "fluorescent cytoprinting", a novel, nondestructive procedure for assessing micro-organ viability in situ. The key feature of fluorescent cytoprinting is that cytotoxic effects are not measured against control cultures but against a baseline provided by a cytoprint of the same culture before drug addition. Using three experimental designs, we tested the potential of the method for clinical applications. The results using 469 human malignant tumors showed that the micro-organ culture assay can distinguish individual tumor chemosensitivity profiles with an overall success rate of 96%. For three commonly used chemotherapeutic drugs, the observed frequency of responding tumors was found to be comparable to previously reported clinical results using single agents. 
653 |a research-article 
653 |a Human Tumor 
653 |a In vitro 
653 |a Chemotherapy 
653 |a Organ Culture 
653 |a Cytotoxicity 
700 1 |a Dickinson, Katherine  |e verfasserin  |4 aut 
773 0 8 |i in  |t In vitro : cellular & developmental biology : journal of the Tissue Culture Association  |d Gaithersbury, Md  |g Vol. 24, No. 11 (1988), p. 1137-1146  |q 24:11<1137-1146  |w (DE-601)JST041821289  |x 0883-8364 
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952 |d 24  |j 1988  |e 11  |h 1137-1146 

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