Biofilm-grown Burkholderia cepacia complex cells survive antibiotic treatment by avoiding production of reactive oxygen species.

The presence of persister cells has been proposed as a factor in biofilm resilience. In the present study we investigated whether persister cells are present in Burkholderia cepacia complex (Bcc) biofilms, what the molecular basis of antimicrobial tolerance in Bcc persisters is, and how persisters c...an be eradicated from Bcc biofilms. After treatment of Bcc biofilms with high concentrations of various antibiotics often a small subpopulation survived. To investigate the molecular mechanism of tolerance in this subpopulation, Burkholderia cenocepacia biofilms were treated with 1024 µg/ml of tobramycin. Using ROS-specific staining and flow cytometry, we showed that tobramycin increased ROS production in treated sessile cells. However, approximately 0.1% of all sessile cells survived the treatment. A transcriptome analysis showed that several genes from the tricarboxylic acid cycle and genes involved in the electron transport chain were downregulated. In contrast, genes from the glyoxylate shunt were upregulated. These data indicate that protection against ROS is important for the survival of persisters. To confirm this, we determined the number of persisters in biofilms formed by catalase mutants. The persister fraction in ΔkatA and ΔkatB biofilms was significantly reduced, confir Ausführliche Beschreibung Beschreibung ausblenden

1. Person:	Heleen Van Acker
Weitere Personen:	Andrea Sass verfasserin; Silvia Bazzini verfasserin; Karen De Roy verfasserin; Claudia Udine verfasserin; Thomas Messiaen verfasserin; Giovanna Riccardi verfasserin; Nico Boon verfasserin; Hans J Nelis verfasserin; Eshwar Mahenthiralingam verfasserin; Tom Coenye verfasserin
Quelle:	In PLoS ONE Weitere Artikel
Format:	Online-Artikel
Sprache:	English
Beschreibung:	Online-Ressource
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